pc3 cat crl 1435 cell lines Search Results


pc3  (ATCC)
99
ATCC pc3
Pc3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pc3+cat+crl+1435+cell+lines/PC-3/pmc11358145-328-12-13
Average 99 stars, based on 1 article reviews
pc3 - by Bioz Stars, 2026-10
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cat atcc crl 1435  (ATCC)
97
ATCC cat atcc crl 1435
Cat Atcc Crl 1435, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pc3+cat+crl+1435+cell+lines/PC-3%3B+Prostate+Adenocarcinoma%3B+Human/pmc09858485-42-17-13
Average 97 stars, based on 1 article reviews
cat atcc crl 1435 - by Bioz Stars, 2026-10
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pc3 cells  (ATCC)
99
ATCC pc3 cells
LNCaP (left) or <t>PC3</t> (right) cells were treated with DMSO or increasing concentrations of thapsigargin, as indicated, in the presence of 2.5 µg/ml PI. A and B. Cell confluence (the proportion of the surface covered by cells, as determined by phase-contrast images) of LNCaP and PC3 cells plotted over time as absolute percentage (A) or relative to start confluence (B). C. The ratio of red fluorescent (PI-positive) to total cell confluence plotted over time. Means ± SEM of 3 (LNCaP) or 6 (PC3) independent experiments.
Pc3 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pc3+cat+crl+1435+cell+lines/LNCaP+clone+FGC/pmc08275286-30-48-50
Average 99 stars, based on 1 article reviews
pc3 cells - by Bioz Stars, 2026-10
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pc-3 cell line crl-1435  (ScienCell)
90
ScienCell pc-3 cell line crl-1435
LNCaP (left) or <t>PC3</t> (right) cells were treated with DMSO or increasing concentrations of thapsigargin, as indicated, in the presence of 2.5 µg/ml PI. A and B. Cell confluence (the proportion of the surface covered by cells, as determined by phase-contrast images) of LNCaP and PC3 cells plotted over time as absolute percentage (A) or relative to start confluence (B). C. The ratio of red fluorescent (PI-positive) to total cell confluence plotted over time. Means ± SEM of 3 (LNCaP) or 6 (PC3) independent experiments.
Pc 3 Cell Line Crl 1435, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pc3+cat+crl+1435+cell+lines/pc+3+cell+line+crl+1435/10__4172_slash_2167___0412__1000256-48-44-56
Average 90 stars, based on 1 article reviews
pc-3 cell line crl-1435 - by Bioz Stars, 2026-10
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293t  (ATCC)
99
ATCC 293t
LNCaP (left) or <t>PC3</t> (right) cells were treated with DMSO or increasing concentrations of thapsigargin, as indicated, in the presence of 2.5 µg/ml PI. A and B. Cell confluence (the proportion of the surface covered by cells, as determined by phase-contrast images) of LNCaP and PC3 cells plotted over time as absolute percentage (A) or relative to start confluence (B). C. The ratio of red fluorescent (PI-positive) to total cell confluence plotted over time. Means ± SEM of 3 (LNCaP) or 6 (PC3) independent experiments.
293t, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pc3+cat+crl+1435+cell+lines/293T/custom%40crl-3216%4040128621
Average 99 stars, based on 1 article reviews
293t - by Bioz Stars, 2026-10
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nci-h460  (ATCC)
98
ATCC nci-h460
LNCaP (left) or <t>PC3</t> (right) cells were treated with DMSO or increasing concentrations of thapsigargin, as indicated, in the presence of 2.5 µg/ml PI. A and B. Cell confluence (the proportion of the surface covered by cells, as determined by phase-contrast images) of LNCaP and PC3 cells plotted over time as absolute percentage (A) or relative to start confluence (B). C. The ratio of red fluorescent (PI-positive) to total cell confluence plotted over time. Means ± SEM of 3 (LNCaP) or 6 (PC3) independent experiments.
Nci H460, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pc3+cat+crl+1435+cell+lines/NCI-H460/custom%40htb-177%4028306514
Average 98 stars, based on 1 article reviews
nci-h460 - by Bioz Stars, 2026-10
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c6  (ATCC)
96
ATCC c6
LNCaP (left) or <t>PC3</t> (right) cells were treated with DMSO or increasing concentrations of thapsigargin, as indicated, in the presence of 2.5 µg/ml PI. A and B. Cell confluence (the proportion of the surface covered by cells, as determined by phase-contrast images) of LNCaP and PC3 cells plotted over time as absolute percentage (A) or relative to start confluence (B). C. The ratio of red fluorescent (PI-positive) to total cell confluence plotted over time. Means ± SEM of 3 (LNCaP) or 6 (PC3) independent experiments.
C6, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pc3+cat+crl+1435+cell+lines/C6/custom%40ccl-107%4016545134
Average 96 stars, based on 1 article reviews
c6 - by Bioz Stars, 2026-10
96/100 stars
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achn  (ATCC)
97
ATCC achn
LNCaP (left) or <t>PC3</t> (right) cells were treated with DMSO or increasing concentrations of thapsigargin, as indicated, in the presence of 2.5 µg/ml PI. A and B. Cell confluence (the proportion of the surface covered by cells, as determined by phase-contrast images) of LNCaP and PC3 cells plotted over time as absolute percentage (A) or relative to start confluence (B). C. The ratio of red fluorescent (PI-positive) to total cell confluence plotted over time. Means ± SEM of 3 (LNCaP) or 6 (PC3) independent experiments.
Achn, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pc3+cat+crl+1435+cell+lines/ACHN/custom%40crl-1611%40pmc12305674__MD-016-D5MD00399G-s001
Average 97 stars, based on 1 article reviews
achn - by Bioz Stars, 2026-10
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u-2 os  (ATCC)
99
ATCC u-2 os
LNCaP (left) or <t>PC3</t> (right) cells were treated with DMSO or increasing concentrations of thapsigargin, as indicated, in the presence of 2.5 µg/ml PI. A and B. Cell confluence (the proportion of the surface covered by cells, as determined by phase-contrast images) of LNCaP and PC3 cells plotted over time as absolute percentage (A) or relative to start confluence (B). C. The ratio of red fluorescent (PI-positive) to total cell confluence plotted over time. Means ± SEM of 3 (LNCaP) or 6 (PC3) independent experiments.
U 2 Os, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pc3+cat+crl+1435+cell+lines/U-2+OS/custom%40htb-96%4039437789
Average 99 stars, based on 1 article reviews
u-2 os - by Bioz Stars, 2026-10
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mda-mb-231  (ATCC)
99
ATCC mda-mb-231
LNCaP (left) or <t>PC3</t> (right) cells were treated with DMSO or increasing concentrations of thapsigargin, as indicated, in the presence of 2.5 µg/ml PI. A and B. Cell confluence (the proportion of the surface covered by cells, as determined by phase-contrast images) of LNCaP and PC3 cells plotted over time as absolute percentage (A) or relative to start confluence (B). C. The ratio of red fluorescent (PI-positive) to total cell confluence plotted over time. Means ± SEM of 3 (LNCaP) or 6 (PC3) independent experiments.
Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pc3+cat+crl+1435+cell+lines/MDA-MB-231/custom%40htb-26%40pmc12305674__MD-016-D5MD00399G-s001
Average 99 stars, based on 1 article reviews
mda-mb-231 - by Bioz Stars, 2026-10
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Image Search Results


LNCaP (left) or PC3 (right) cells were treated with DMSO or increasing concentrations of thapsigargin, as indicated, in the presence of 2.5 µg/ml PI. A and B. Cell confluence (the proportion of the surface covered by cells, as determined by phase-contrast images) of LNCaP and PC3 cells plotted over time as absolute percentage (A) or relative to start confluence (B). C. The ratio of red fluorescent (PI-positive) to total cell confluence plotted over time. Means ± SEM of 3 (LNCaP) or 6 (PC3) independent experiments.

Journal: Bio-protocol

Article Title: An Image-based Assay for High-throughput Analysis of Cell Proliferation and Cell Death of Adherent Cells

doi: 10.21769/BioProtoc.2835

Figure Lengend Snippet: LNCaP (left) or PC3 (right) cells were treated with DMSO or increasing concentrations of thapsigargin, as indicated, in the presence of 2.5 µg/ml PI. A and B. Cell confluence (the proportion of the surface covered by cells, as determined by phase-contrast images) of LNCaP and PC3 cells plotted over time as absolute percentage (A) or relative to start confluence (B). C. The ratio of red fluorescent (PI-positive) to total cell confluence plotted over time. Means ± SEM of 3 (LNCaP) or 6 (PC3) independent experiments.

Article Snippet: In the current protocol we use: 96-well tissue culture plates (Corning, Falcon ® , catalog number: 353072) Sterile 50 ml tubes (VWR, catalog number: 525-0402) 0.2 µm filter with PES membranes (VWR, catalog number: 514-0073) 50 ml syringe (VWR, catalog number: 613-2053) LNCaP cells (ATCC, catalog number: CRL-1740) PC3 cells (ATCC, catalog number: CRL-1435) RPMI1640 medium (Thermo Fisher Scientific, Gibco TM , catalog number: 21875) Fetal bovine serum (Sigma-Aldrich, catalog number: F7524; use at 10% final concentration in RPMI 1640 medium to make complete growth medium) Dimethyl sulfoxide (DMSO) (Sigma-Aldrich, catalog number: D2650) 0.25% Trypsin-EDTA (1x) (Thermo Fisher Scientific, Gibco TM , catalog number: 25200056) PBS (Thermo Fisher Scientific, Gibco TM , catalog number: 20012) Propidium Iodide (Merck, Calbiochem ® , catalog number: 537059) Thapsigargin (Sigma-Aldrich, catalog number: T9033) Propidium iodide, 1 mg/ml stock solution in PBS (see Recipes) Thapsigargin (Tg), 5 mM stock solution in DMSO (see Recipes)

Techniques:

Overlayed phase-contrast and fluorescence images (10x magnification) of LNCaP (A) and PC3 (B) cells after 0 h, 24 h, 48 h and 72 h of treatment with DMSO or thapsigargin (Tg), in the presence of PI. Scale bar represents 300 µm.

Journal: Bio-protocol

Article Title: An Image-based Assay for High-throughput Analysis of Cell Proliferation and Cell Death of Adherent Cells

doi: 10.21769/BioProtoc.2835

Figure Lengend Snippet: Overlayed phase-contrast and fluorescence images (10x magnification) of LNCaP (A) and PC3 (B) cells after 0 h, 24 h, 48 h and 72 h of treatment with DMSO or thapsigargin (Tg), in the presence of PI. Scale bar represents 300 µm.

Article Snippet: In the current protocol we use: 96-well tissue culture plates (Corning, Falcon ® , catalog number: 353072) Sterile 50 ml tubes (VWR, catalog number: 525-0402) 0.2 µm filter with PES membranes (VWR, catalog number: 514-0073) 50 ml syringe (VWR, catalog number: 613-2053) LNCaP cells (ATCC, catalog number: CRL-1740) PC3 cells (ATCC, catalog number: CRL-1435) RPMI1640 medium (Thermo Fisher Scientific, Gibco TM , catalog number: 21875) Fetal bovine serum (Sigma-Aldrich, catalog number: F7524; use at 10% final concentration in RPMI 1640 medium to make complete growth medium) Dimethyl sulfoxide (DMSO) (Sigma-Aldrich, catalog number: D2650) 0.25% Trypsin-EDTA (1x) (Thermo Fisher Scientific, Gibco TM , catalog number: 25200056) PBS (Thermo Fisher Scientific, Gibco TM , catalog number: 20012) Propidium Iodide (Merck, Calbiochem ® , catalog number: 537059) Thapsigargin (Sigma-Aldrich, catalog number: T9033) Propidium iodide, 1 mg/ml stock solution in PBS (see Recipes) Thapsigargin (Tg), 5 mM stock solution in DMSO (see Recipes)

Techniques: Fluorescence

PC3 cells were treated for 42 h with DMSO or increasing concentrations of thapsigargin, as indicated, in the presence of PI. Cell death was first analyzed by live-cell imaging using the IncuCyte ZOOM (A), and subsequently by flow cytometry (using BD Bioscience FACSCanto II and the Flowing Software by Perttu Terho) from the same wells (B). For flow cytometry, the cells were harvested with 0.25% Trypsin-EDTA (1x), and 10,000 events were acquired and analyzed as previously described ( Crowley et al., 2016 ). Cellular debris were excluded by gating forward/side scatter plots, and PI-positive cells were gated based on forward scatter/PI fluorescence plots, as described ( Crowley et al., 2016 ). Shown are ratios of red fluorescence to total cell confluence (A) and percentages of gated PI-positive cells (B). Means ± SEM of 3 independent experiments. EC50 values of the maximal thapsigargin effect (with 95% Confidence Intervals) are shown.

Journal: Bio-protocol

Article Title: An Image-based Assay for High-throughput Analysis of Cell Proliferation and Cell Death of Adherent Cells

doi: 10.21769/BioProtoc.2835

Figure Lengend Snippet: PC3 cells were treated for 42 h with DMSO or increasing concentrations of thapsigargin, as indicated, in the presence of PI. Cell death was first analyzed by live-cell imaging using the IncuCyte ZOOM (A), and subsequently by flow cytometry (using BD Bioscience FACSCanto II and the Flowing Software by Perttu Terho) from the same wells (B). For flow cytometry, the cells were harvested with 0.25% Trypsin-EDTA (1x), and 10,000 events were acquired and analyzed as previously described ( Crowley et al., 2016 ). Cellular debris were excluded by gating forward/side scatter plots, and PI-positive cells were gated based on forward scatter/PI fluorescence plots, as described ( Crowley et al., 2016 ). Shown are ratios of red fluorescence to total cell confluence (A) and percentages of gated PI-positive cells (B). Means ± SEM of 3 independent experiments. EC50 values of the maximal thapsigargin effect (with 95% Confidence Intervals) are shown.

Article Snippet: In the current protocol we use: 96-well tissue culture plates (Corning, Falcon ® , catalog number: 353072) Sterile 50 ml tubes (VWR, catalog number: 525-0402) 0.2 µm filter with PES membranes (VWR, catalog number: 514-0073) 50 ml syringe (VWR, catalog number: 613-2053) LNCaP cells (ATCC, catalog number: CRL-1740) PC3 cells (ATCC, catalog number: CRL-1435) RPMI1640 medium (Thermo Fisher Scientific, Gibco TM , catalog number: 21875) Fetal bovine serum (Sigma-Aldrich, catalog number: F7524; use at 10% final concentration in RPMI 1640 medium to make complete growth medium) Dimethyl sulfoxide (DMSO) (Sigma-Aldrich, catalog number: D2650) 0.25% Trypsin-EDTA (1x) (Thermo Fisher Scientific, Gibco TM , catalog number: 25200056) PBS (Thermo Fisher Scientific, Gibco TM , catalog number: 20012) Propidium Iodide (Merck, Calbiochem ® , catalog number: 537059) Thapsigargin (Sigma-Aldrich, catalog number: T9033) Propidium iodide, 1 mg/ml stock solution in PBS (see Recipes) Thapsigargin (Tg), 5 mM stock solution in DMSO (see Recipes)

Techniques: Live Cell Imaging, Flow Cytometry, Software, Fluorescence

Screenshots of the IncuCyte ZOOM software Analyzer tool for confluence mask (yellow outlines). A. Appropriate adjustment for PC3 cells with segmentation adjustment of 1.8 towards cells (red rectangle) and Filter for areas smaller than 500 µm2 (blue rectangle). B. Example of inappropriate adjustment for PC3 cells with segmentation adjustment of 0.2 towards background (red rectangle) and no Filter (blue rectangle). Marked with arrows are examples of cells/parts of cells which are not included in the confluence mask (red arrows) and debris which are not filtered out (blue arrows). Due to incorrect segmentation adjustment the calculated confluence is too low (9% instead of 39%).

Journal: Bio-protocol

Article Title: An Image-based Assay for High-throughput Analysis of Cell Proliferation and Cell Death of Adherent Cells

doi: 10.21769/BioProtoc.2835

Figure Lengend Snippet: Screenshots of the IncuCyte ZOOM software Analyzer tool for confluence mask (yellow outlines). A. Appropriate adjustment for PC3 cells with segmentation adjustment of 1.8 towards cells (red rectangle) and Filter for areas smaller than 500 µm2 (blue rectangle). B. Example of inappropriate adjustment for PC3 cells with segmentation adjustment of 0.2 towards background (red rectangle) and no Filter (blue rectangle). Marked with arrows are examples of cells/parts of cells which are not included in the confluence mask (red arrows) and debris which are not filtered out (blue arrows). Due to incorrect segmentation adjustment the calculated confluence is too low (9% instead of 39%).

Article Snippet: In the current protocol we use: 96-well tissue culture plates (Corning, Falcon ® , catalog number: 353072) Sterile 50 ml tubes (VWR, catalog number: 525-0402) 0.2 µm filter with PES membranes (VWR, catalog number: 514-0073) 50 ml syringe (VWR, catalog number: 613-2053) LNCaP cells (ATCC, catalog number: CRL-1740) PC3 cells (ATCC, catalog number: CRL-1435) RPMI1640 medium (Thermo Fisher Scientific, Gibco TM , catalog number: 21875) Fetal bovine serum (Sigma-Aldrich, catalog number: F7524; use at 10% final concentration in RPMI 1640 medium to make complete growth medium) Dimethyl sulfoxide (DMSO) (Sigma-Aldrich, catalog number: D2650) 0.25% Trypsin-EDTA (1x) (Thermo Fisher Scientific, Gibco TM , catalog number: 25200056) PBS (Thermo Fisher Scientific, Gibco TM , catalog number: 20012) Propidium Iodide (Merck, Calbiochem ® , catalog number: 537059) Thapsigargin (Sigma-Aldrich, catalog number: T9033) Propidium iodide, 1 mg/ml stock solution in PBS (see Recipes) Thapsigargin (Tg), 5 mM stock solution in DMSO (see Recipes)

Techniques: Software